Diversity of the bovine genes IRAK1 and IRAK4 in the Toll-like receptor signaling pathway

Innate immunity is the organism’s first line of defence, and receptors immune cells play a key role. Interleukin 1 receptor-associated kinase 4 (IRAK1 IRAK4) are necessary for signal transmission from Toll-like receptors, which most important cells. The main objective this study was to investigate gene polymorphisms IRAK1 IRAK4 in Czech Fleckvieh cattle (CF) to compare their diversity with other breeds as there still little information about these genes. PacBio Technology next-generation sequencing (NGS) was used detect genetic variability 164 CF bulls. results revealed 17 single nucleotide variants (SNVs) the IRAK1 12 SNVs three deletions gene. Three methods were then determine allelic variability: Sanger sequencing, primer extension method Illumina DNA BeadChip microarray cooperation Moravian Breeder’s Corporation. enabled analysis population (SNPs) rs210710958 rs48726521 SNaPshot genotyping employed frequencies SNP rs211379365 novel polymorphism IRAK1, rs380202447 method, applied 74 dairy cows, frequency rs110533802 including 0.07 mutant allele T. Additional data allowed comparison among Hungarian populations Holstein cows beef breed populations. show that functionally significant and IRAK4 genes studied rather limited. higher 0.4 T could be consequence breeding milk performance.